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Fig. 5 | BMC Molecular and Cell Biology

Fig. 5

From: Substance P enhances the local activation of NK1R-expressing c-kit+ cardiac progenitor cells in right atrium of ischemia/reperfusion-injured heart

Fig. 5

Co-expression of c-Kit and NK1R in RA1 day I/R and RA1 day SP + I/R EDCs. (A) Confocal fluorescence images of NK1R-expressing c-Kit+ CPCs in RA1 day 1/R EDCs and RA1 day SP + 1/R EDCs. c-Kit (green), NK1R (Red), Hoechst33342 nuclei (blue) are visualized. Scale bars, 20 μm. (B) Western blot analysis to detect the expression of proteins NK1R and c-Kit in each group. Actin was used as a loading control. (C and D) qRT-PCR analysis graphs showing the mRNA expression of NK1R and c-Kit in RA1 day 1/R EDCs and RA1 day SP + 1/R EDCs. The bars represent the mean ± SD of triplicate assays expressed as percentages of the RA1 day I/R EDCs. *P < 0.05 and **P < 0.01 versus corresponding controls

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BMC Molecular and Cell Biology

ISSN: 2661-8850

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