Fig. 3

The calibration of the spatial framework and predictions on the colony morphology and colony composition. The colony composition is illustrated for a quarter colony which contains full information of the symmetric colony. a Simulated colony footprint areas for wild type and petite strain are plotted using black and red dashed lines, respectively. Experimental data from wild type and petite strains (three replicates from both strains) are plotted using black and red solid lines, respectively. The black arrow indicates the wild type replicate which was used to calibrate the model. The data from the petite strain is used only for validation purposes. b Isosurface illustration of the simulated colony shape and cell state composition at time 121 hours. c Simulated cell state and nutrient distributions for wild type and petite strains at time 121 hours illustrated using heatmaps. The shown vertical slice is located in the middle of the colony. d Simulated time-evolution of all model component all total cell mass at different spatial locations. The exact coornitates (in mm) for illustrated point are (1,1,1),(0.1,0.1,1.0),(0.1,1.5,0.2), and (0.1,0.1,0.1) (starting from the upper row)