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Figure 2 | BMC Cell Biology

Figure 2

From: Copine A, a calcium-dependent membrane-binding protein, transiently localizes to the plasma membrane and intracellular vacuoles in Dictyostelium

Figure 2

CpnA is present throughout development. Wildtype Dictyostelium cells in starvation buffer were placed on filters to develop. At 5-hour intervals, protein and RNA samples were harvested. A) Whole cell protein samples (5 × 106 cells per lane) from each time point were analyzed by Western blot using a purified CpnA antibody (arrow points to the 66 kDa CpnA). B) RNA samples from each time point were used in RT-PCR to detect cpnA mRNA. RT-PCR without the RT was run as a control (no RT). C) Whole cell protein samples (5 × 106 cells per lane) from wildtype cells and cpnA- cells were analyzed by Western blot using the CpnA antisera (arrow points to the 66 kDa CpnA).

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BMC Molecular and Cell Biology

ISSN: 2661-8850

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