Figure 2
From: Dynamic assembly, localization and proteolysis of the Bacillus subtilis SMC complex
SDS PAGE showing fractions eluted after sucrose gradient centrifugation using 5 to 20% sucrose concentration. 0.4 nmol of purified ScpB (upper panel, Coomassie stained), cell extract probed with ScpB antiserum (middle panel) or with SMC antiserum (lower panel). Lower and middle panels are Western blots. Arrowheads indicate the elution peaks for ScpB (grey) or for SMC (white). Sedimentation peaks of marker proteins are shown on the top.