Your privacy, your choice

We use essential cookies to make sure the site can function. We also use optional cookies for advertising, personalisation of content, usage analysis, and social media.

By accepting optional cookies, you consent to the processing of your personal data - including transfers to third parties. Some third parties are outside of the European Economic Area, with varying standards of data protection.

See our privacy policy for more information on the use of your personal data.

for further information and to change your choices.

Skip to main content
Figure 2 | BMC Cell Biology

Figure 2

From: Activation of α1A-adrenergic receptor promotes differentiation of rat-1 fibroblasts to a smooth muscle-like phenotype

Figure 2

Stimulation of α 1A -AR mediates the effect of PE on DNA and protein synthesis. A, Effect of prazosin (α1-AR antagonist) and propranolol (β-AR antagonist) on PE-induced inhibition of DNA synthesis. Cells were treated with prazosin and/or propranolol for 30 min before the addition of 5 μM PE for 18 h. [3H]thymidine incorporation was measured as described in Methods. Data are expressed as dpm of [3H]thymidine incorporated per well. Values are the mean ± S.E. of three independent experiments performed in quadruplicates on different batches of cells. * Value significantly different from the corresponding value obtained without PE treatment, p < .05. B, Effect of prazosin and propranolol on PE-induced increase in protein synthesis. Experimental conditions were similar as described in A. [3H]leucine incorporation was measured as described in Methods. Data are expressed as dpm of [3H]leucine incorporated per well. Values are the mean ± S.E of three independent experiments performed in quadruplicates on different batches of cells. * Value significantly different from the corresponding value obtained without PE treatment, p < .05.

Back to article page