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Figure 2 | BMC Cell Biology

Figure 2

From: Intracellular shuttling of a Drosophila APC tumour suppressor homolog

Figure 2

FRAP protocol for the analysis of live Drosophila embryos expressing GFP-E-APC. (a) Sketch of the microscopy and data analysis used to determine the mobility of GFP-E-APC in epithelial cells of live embryos. (b) Consecutive face-on views of a live ~6 hours old embryo (stage 11) expressing GFP-E-APC, with squares marking specific sections of the AJs at cell interfaces; the set of yellow squares in the right-hand image illustrate the cell shape changes that took place during the 5 minutes between the two optical sections shown. White bar in this and subsequent figures, 5 μm.

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BMC Molecular and Cell Biology

ISSN: 2661-8850

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